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Communication
Generating DNA Synbodies from Previously Discovered Peptides
Article first published online: 20 JUN 2011
DOI: 10.1002/cbic.201100284
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Liu, R., Jiang, B., Yu, H. and Chaput, J. C. (2011), Generating DNA Synbodies from Previously Discovered Peptides. ChemBioChem, 12: 1813–1817. doi: 10.1002/cbic.201100284
Publication History
- Issue published online: 5 AUG 2011
- Article first published online: 20 JUN 2011
- Manuscript Received: 5 MAY 2011
Funded by
- National Institutes of Health. Grant Number: CA126622-01
- Science Foundation of Arizona. Grant Number: CAA 0265-08
- National Cancer Institute
- Abstract
- Article
- References
- Cited By
Keywords:
- antibodies;
- Grb2;
- growth factors;
- synthetic antibodies;
- synthetic biology
Synbody building: We demonstrate how off-the-shelf peptides can be transformed into high affinity protein capture reagents that mimic the recognition properties of natural antibodies (see figure). The designer synthetic antibody amplifies the binding affinity of the individual peptides by ∼1000-fold to bind Grb2 with a Kd of 2 nM, and functions with high selectivity in conventional pull-down assays from HeLa cell lysates.