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Keywords:

  • antibiotics;
  • bioinorganic chemistry;
  • lactams;
  • metalloenzymes;
  • UV/Vis spectroscopy
Thumbnail image of graphical abstract

Think zinc: We have developed a new preparation method for generating the apo-enzyme of IMP-1 metallo-β-lactamase (apo-IMP-1) with the combination of a chelating agent (EDTA) and desalting column chromatography. An advantage of this method is that enzymatic activity of the apo-enzyme comparable to that observed with the native enzyme could be restored upon addition of ZnII ions (see scheme).