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Keywords:

  • biosynthesis;
  • fusion proteins;
  • lasso peptides;
  • microcin J25;
  • peptides
Thumbnail image of graphical abstract

Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter (see graphic). We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.