Communication

Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25

  1. Si Jia Pan1,
  2. Jakub Rajniak1,
  3. Wai Ling Cheung1,
  4. Prof. A. James Link1,2,*

Article first published online: 23 DEC 2011

DOI: 10.1002/cbic.201100596

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 3, pages 367–370, February 13, 2012

Additional Information

How to Cite

Pan, S. J., Rajniak, J., Cheung, W. L. and Link, A. J. (2012), Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25. ChemBioChem, 13: 367–370. doi: 10.1002/cbic.201100596

Author Information

  1. 1

    Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544 (USA)

  2. 2

    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 (USA)

*Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544 (USA)

Publication History

  1. Issue published online: 3 FEB 2012
  2. Article first published online: 23 DEC 2011
  3. Manuscript Received: 21 SEP 2011

Funded by

  • NSF. Grant Number: CBET-0952875

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (330K)

Keywords:

  • biosynthesis;
  • fusion proteins;
  • lasso peptides;
  • microcin J25;
  • peptides
Thumbnail image of graphical abstract

Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter (see graphic). We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.

View Full Article with Supporting Information (HTML) Get PDF (330K)