Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25

Authors

  • Si Jia Pan,

    1. Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544 (USA)
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  • Jakub Rajniak,

    1. Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544 (USA)
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  • Wai Ling Cheung,

    1. Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544 (USA)
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  • Prof. A. James Link

    Corresponding author
    1. Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544 (USA)
    2. Department of Molecular Biology, Princeton University, Princeton, NJ 08544 (USA)
    • Department of Chemical and Biological Engineering, Princeton University, A207 Engineering Quadrangle, Princeton, NJ 08544 (USA)
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Abstract

original image

Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter (see graphic). We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.

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