Kinetic Capillary Electrophoresis with Mass-Spectrometry Detection (KCE-MS) Facilitates Label-Free Solution-Based Kinetic Analysis of Protein–Small Molecule Binding

Authors

  • Jiayin Bao,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Dr. Svetlana M. Krylova,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Prof. Derek J. Wilson,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Oren Reinstein,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Prof. Dr. Philip E. Johnson,

    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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  • Prof. Dr. Sergey N. Krylov

    Corresponding author
    1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
    • Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)
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Abstract

original image

Tandem tracker: Here we introduce a method for studying the kinetics of protein–small-molecule interactions based on kinetic capillary electrophoresis (KCE) separation and MS detection. Due to the variety of KCE methods and MS modes available, the KCE-MS tandem is a highly versatile platform for label-free, solution-based kinetic studies of affinity interactions.

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