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Communication

Kinetic Capillary Electrophoresis with Mass-Spectrometry Detection (KCE-MS) Facilitates Label-Free Solution-Based Kinetic Analysis of Protein–Small Molecule Binding

  1. Jiayin Bao,
  2. Dr. Svetlana M. Krylova,
  3. Prof. Derek J. Wilson,
  4. Oren Reinstein,
  5. Prof. Dr. Philip E. Johnson,
  6. Prof. Dr. Sergey N. Krylov*

Article first published online: 19 OCT 2011

DOI: 10.1002/cbic.201100617

Issue

ChemBioChem

ChemBioChem

Volume 12, Issue 17, pages 2551–2554, November 25, 2011

Additional Information

How to Cite

Bao, J., Krylova, S. M., Wilson, D. J., Reinstein, O., Johnson, P. E. and Krylov, S. N. (2011), Kinetic Capillary Electrophoresis with Mass-Spectrometry Detection (KCE-MS) Facilitates Label-Free Solution-Based Kinetic Analysis of Protein–Small Molecule Binding. ChemBioChem, 12: 2551–2554. doi: 10.1002/cbic.201100617

Author Information

  1. Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)

*Department of Chemistry and Centre for Research on Biomolecular Interactions, York University 4700 Keele Street, Toronto, Ontario M3J 1P3 (Canada)

Publication History

  1. Issue published online: 18 NOV 2011
  2. Article first published online: 19 OCT 2011
  3. Manuscript Received: 29 SEP 2011

Funded by

  • NSERC Canada

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Keywords:

  • electrophoresis;
  • label-free kinetics assays;
  • mass spectrometry;
  • proteins;
  • small molecules
Thumbnail image of graphical abstract

Tandem tracker: Here we introduce a method for studying the kinetics of protein–small-molecule interactions based on kinetic capillary electrophoresis (KCE) separation and MS detection. Due to the variety of KCE methods and MS modes available, the KCE-MS tandem is a highly versatile platform for label-free, solution-based kinetic studies of affinity interactions.

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