Communication

Expansion of the Amino Acid Repertoire in Protein Biosynthesis in Silkworm Cells

  1. Dr. Hidetoshi Teramoto1,*,
  2. Dr. Katsura Kojima1,
  3. Dr. Hideyuki Kajiwara2,
  4. Dr. Jun Ishibashi1

Article first published online: 24 NOV 2011

DOI: 10.1002/cbic.201100624

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 1, pages 61–65, January 2, 2012

Additional Information

How to Cite

Teramoto, H., Kojima, K., Kajiwara, H. and Ishibashi, J. (2012), Expansion of the Amino Acid Repertoire in Protein Biosynthesis in Silkworm Cells. ChemBioChem, 13: 61–65. doi: 10.1002/cbic.201100624

Author Information

  1. 1

    Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634 (Japan)

  2. 2

    Agrogenomics Research Center, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602 (Japan)

*Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634 (Japan)

Publication History

  1. Issue published online: 22 DEC 2011
  2. Article first published online: 24 NOV 2011
  3. Manuscript Received: 4 OCT 2011

Funded by

  • Ministry of Education, Culture, Sports, Science and Technology of Japan. Grant Numbers: 20780039, 22658019

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Keywords:

  • amino acids;
  • aminoacyl-tRNA synthetases;
  • protein engineering;
  • silkworms;
  • unnatural amino acids
Thumbnail image of graphical abstract

Toward silk production with UAAs: The strategy for residue-specific incorporation of unnatural amino acids (UAAs) into proteins was extended to silkworm cells. Here we report the incorporation of p-Cl-Phe into a reporter protein (EGFP) synthesized in silkworm cells that express an αA450G mutant of B. mori phenylalanyl-tRNA synthetase with relaxed substrate specificity.

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