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Imaging the Sialome during Zebrafish Development with Copper-Free Click Chemistry

  1. Dr. Karen W. Dehnert1,
  2. Dr. Jeremy M. Baskin1,
  3. Dr. Scott T. Laughlin1,
  4. Brendan J. Beahm1,
  5. Natasha N. Naidu2,
  6. Prof. Sharon L. Amacher3,
  7. Prof. Carolyn R. Bertozzi1,3,4,*

Article first published online: 20 JAN 2012

DOI: 10.1002/cbic.201100649

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 3, pages 353–357, February 13, 2012

Additional Information

How to Cite

Dehnert, K. W., Baskin, J. M., Laughlin, S. T., Beahm, B. J., Naidu, N. N., Amacher, S. L. and Bertozzi, C. R. (2012), Imaging the Sialome during Zebrafish Development with Copper-Free Click Chemistry. ChemBioChem, 13: 353–357. doi: 10.1002/cbic.201100649

Author Information

  1. 1

    Department of Chemistry, University of California, B84 Hildebrand Hall 1460, Berkeley, CA 94720 (USA)

  2. 2

    Glycotechnology Core Resource, University of California, San Diego, La Jolla, CA 92093 (USA)

  3. 3

    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 (USA)

  4. 4

    Howard Hughes Medical Institute, University of California, Berkeley, CA 94720 (USA)

*Department of Chemistry, University of California, B84 Hildebrand Hall 1460, Berkeley, CA 94720 (USA)

Publication History

  1. Issue published online: 3 FEB 2012
  2. Article first published online: 20 JAN 2012
  3. Manuscript Received: 17 OCT 2011

Funded by

  • National Institutes of Health. Grant Numbers: GM58867, GM61952
  • National Science Foundation
  • National Defense Science & Engineering

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Keywords:

  • bioorthogonal chemistry;
  • embryonic development;
  • fluorescence imaging;
  • glycosylation;
  • sialic acids
Thumbnail image of graphical abstract

The sialome comprises sialylated glycoproteins and glycolipids that play essential roles in cell–cell communication. Using azide-modified molecular precursors of sialic acids and copper-free click chemistry, we visualized the spatiotemporal dynamics of the sialome in live zebrafish embryos.

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