Live-Cell dSTORM of Cellular DNA Based on Direct DNA Labeling

Authors

  • Alexander Benke,

    1. Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, 1005 Lausanne (Switzerland)
    Search for more papers by this author
  • Prof. Suliana Manley

    Corresponding author
    1. Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, 1005 Lausanne (Switzerland)
    • Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, 1005 Lausanne (Switzerland)
    Search for more papers by this author

Abstract

original image

We have implemented the super-resolution method of direct stochastic optical reconstruction microscopy (dSTORM) to image nuclear and mitochondrial DNA in living cells. We also demonstrate time-lapse imaging, all using a dye that associates directly with cellular DNA: the commercially available dye Picogreen (see figure).

Ancillary