Communication

Stereospecific Formation of a Ternary Complex of (S)-α,β-Fluoromethylene-dATP with DNA Pol β

  1. Brian T. Chamberlain2,
  2. Dr. Vinod K. Batra1,
  3. Dr. William A. Beard1,
  4. Anastasia P. Kadina2,
  5. David D. Shock1,
  6. Dr. Boris A. Kashemirov2,
  7. Dr. Charles E. McKenna2,*,
  8. Dr. Myron F. Goodman2,
  9. Dr. Samuel H. Wilson1,*

Article first published online: 7 FEB 2012

DOI: 10.1002/cbic.201100738

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 4, pages 528–530, March 5, 2012

Additional Information

How to Cite

Chamberlain, B. T., Batra, V. K., Beard, W. A., Kadina, A. P., Shock, D. D., Kashemirov, B. A., McKenna, C. E., Goodman, M. F. and Wilson, S. H. (2012), Stereospecific Formation of a Ternary Complex of (S)-α,β-Fluoromethylene-dATP with DNA Pol β. ChemBioChem, 13: 528–530. doi: 10.1002/cbic.201100738

Author Information

  1. 1

    Laboratory of Structural Biology, NIEHS, National Institutes of Health, DHHS, Research Triangle Park, NC 27709 (USA)

  2. 2

    Departments of Chemistry and Biology, University of Southern California, Los Angeles, CA 90089 (USA)

*Departments of Chemistry and Biology, University of Southern California, Los Angeles, CA 90089 (USA)

Publication History

  1. Issue published online: 29 FEB 2012
  2. Article first published online: 7 FEB 2012
  3. Manuscript Received: 23 NOV 2011

Funded by

  • NIH. Grant Numbers: U19CA105010, Z01ES050158

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Keywords:

  • enzymes;
  • fluorine;
  • inhibitors;
  • nucleotides
Thumbnail image of graphical abstract

The influence of water: Crystallization of (R/S)-α,β-CHF-dATP with the preorganized pol β-DNA complex shows that (S)-α,β-CHF-dATP is preferentially bound to the active site with the C[BOND]F fluorine proximal to a structural water bound to Asp276.

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