Communication

KlenTaq DNA Polymerase Adopts Unique Recognition States when Encountering Matched, Mismatched, and Abasic Template Sites: An NMR Study

  1. Bastian Holzberger,
  2. M. Gabriele Pszolla,
  3. Prof. Dr. Andreas Marx,
  4. Dr. Heiko M. Möller*

Article first published online: 7 FEB 2012

DOI: 10.1002/cbic.201100802

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 5, pages 635–639, March 19, 2012

Additional Information

How to Cite

Holzberger, B., Pszolla, M. G., Marx, A. and Möller, H. M. (2012), KlenTaq DNA Polymerase Adopts Unique Recognition States when Encountering Matched, Mismatched, and Abasic Template Sites: An NMR Study. ChemBioChem, 13: 635–639. doi: 10.1002/cbic.201100802

Author Information

  1. Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany)

*Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany)

Publication History

  1. Issue published online: 15 MAR 2012
  2. Article first published online: 7 FEB 2012
  3. Manuscript Received: 26 DEC 2011

Funded by

  • Konstanz Research School Chemical Biology
  • DFG

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Keywords:

  • abasic sites;
  • DNA polymerases;
  • DNA replication;
  • mismatches;
  • NMR spectroscopy
Thumbnail image of graphical abstract

On the tracks of a DNA polymerase: NMR provides insights into DNA synthesis in a virtually label-free manner and under close-to-physiological conditions. Through the monitoring of the chemical-shift changes of multiple 13C-methyl methionine residues we found unique recognition states for canonical and noncanonical cases, thus indicating enzymatic cycling through distinct paths.

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