Cover Picture

Cover Picture: Bromomaleimide-Linked Bioconjugates Are Cleavable in Mammalian Cells (ChemBioChem 1/2012)

  1. Paul Moody1,2,
  2. Dr. Mark. E. B. Smith1,
  3. Dr. Chris P. Ryan1,
  4. Dr. Vijay Chudasama1,
  5. Dr. James R. Baker1,*,
  6. Dr. Justin Molloy2,*,
  7. Prof. Stephen Caddick1,*

Article first published online: 22 DEC 2011

DOI: 10.1002/cbic.201190090

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 1, page 1, January 2, 2012

Additional Information

How to Cite

Moody, P., Smith, Mark. E. B., Ryan, C. P., Chudasama, V., Baker, J. R., Molloy, J. and Caddick, S. (2012), Cover Picture: Bromomaleimide-Linked Bioconjugates Are Cleavable in Mammalian Cells (ChemBioChem 1/2012). ChemBioChem, 13: 1. doi: 10.1002/cbic.201190090

Author Information

  1. 1

    Department of Chemistry, University College London, Gordon Street, London, WC1H 0AJ (UK)

  2. 2

    MRC National Institute of Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA (UK)

*Department of Chemistry, University College London, Gordon Street, London, WC1H 0AJ (UK)

Publication History

  1. Issue published online: 22 DEC 2011
  2. Article first published online: 22 DEC 2011

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Keywords:

  • drug delivery;
  • fluorescent probes;
  • in-cell cleavage;
  • microinjection;
  • protein modifications
Thumbnail image of graphical abstract

The cover picture shows a conjugate formed between a dibromomaleimide–rhodamine scaffold and two molecules of green fluorescent protein. Bromomaleimide scaffolds may be used for the synthesis of multifunctionalised biomolecules, and it has been postulated that bromomaleimide adducts are cleaved inside cells. On p. 39 ff., J. R. Baker, J. Molloy, S. Caddick et al. now describe the synthesis of bromomaleimide-based FRET constructs that upon microinjection into HeLa cells cleave to release free GFP. This process was monitored by quantitative dual-channel fluorescence video microscopy. Bromomaleimide constructs could therefore be used as cleavable drug delivery systems, or as ratiometric sensors of cytoplasmic delivery. (Cover art designed by Hayley Wood, MRC NIMR, and Dr. Rachel Morgan, UCL).

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