Communication

Site-Selective Affinity Labelling of Maltose Binding Protein in Bacterial Cells

  1. Dr. Anthony Rullo,
  2. Andrew A. Beharry,
  3. Rodolfo F. Gómez-Biagi,
  4. Xiong Zhao,
  5. Prof. Mark Nitz*

Article first published online: 29 FEB 2012

DOI: 10.1002/cbic.201200046

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 6, pages 783–787, April 16, 2012

Additional Information

How to Cite

Rullo, A., Beharry, A. A., Gómez-Biagi, R. F., Zhao, X. and Nitz, M. (2012), Site-Selective Affinity Labelling of Maltose Binding Protein in Bacterial Cells. ChemBioChem, 13: 783–787. doi: 10.1002/cbic.201200046

Author Information

  1. Department of Chemistry, University of Toronto, Toronto, ON, Canada M5S 3H6 (Canada)

*Department of Chemistry, University of Toronto, Toronto, ON, Canada M5S 3H6 (Canada)

Publication History

  1. Issue published online: 11 APR 2012
  2. Article first published online: 29 FEB 2012
  3. Manuscript Received: 18 JAN 2012

Funded by

  • Natural Sciences and Engineering Research Council of Canada (NSERC)

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Keywords:

  • bioorganic chemistry;
  • glycoconjugates;
  • protein conjugation;
  • protein modifications;
  • thioesters
Thumbnail image of graphical abstract

Labelling simplified: In two steps, a thioester carbohydrate conjugate can be synthesised that site-specifically labels maltose binding protein (shown in grey). Specific labelling is observed in crude cell extracts and within live E. coli cells.

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