Communication

Chemical Reporters for Monitoring RNA Synthesis and Poly(A) Tail Dynamics

  1. Markus Grammel2,
  2. Howard Hang2,*,
  3. Nicholas K. Conrad1,*

Article first published online: 19 APR 2012

DOI: 10.1002/cbic.201200091

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 8, pages 1112–1115, May 29, 2012

Additional Information

How to Cite

Grammel, M., Hang, H. and Conrad, N. K. (2012), Chemical Reporters for Monitoring RNA Synthesis and Poly(A) Tail Dynamics. ChemBioChem, 13: 1112–1115. doi: 10.1002/cbic.201200091

Author Information

  1. 1

    Department of Microbiology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390-9048 (USA)

  2. 2

    Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue Box 250, New York, NY 10065 (USA)

*Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue Box 250, New York, NY 10065 (USA)

Publication History

  1. Issue published online: 23 MAY 2012
  2. Article first published online: 19 APR 2012
  3. Manuscript Received: 5 FEB 2012

Funded by

  • NIH/NIAID. Grant Number: AI081710
  • Cancer Prevention and Research Institute of Texas. Grant Number: RP110132
  • Irma T. Hirschl/Monique Weill–Caulier Trust, Lerner Trust. Grant Number: 1R01m087544

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Keywords:

  • cycloaddition;
  • metabolic labeling;
  • molecular biology;
  • nucleosides;
  • RNA
Thumbnail image of graphical abstract

A versatile “clickable” nucleoside: Metabolic labeling of cells is useful in studying the dynamics of biological molecules. N6pA can be utilized by all three mammalian RNA polymerases, as well as poly(A) polymerase. Because of its alkyne modification, RNA labeled with N6pA can be visualized and purified by using click chemistry.

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