Communication

Metabolic Labeling of DNA by Purine Analogues in Vivo

  1. Anne B. Neef,
  2. Dr. Florent Samain,
  3. Prof. Dr. Nathan W. Luedtke*

Article first published online: 20 JUL 2012

DOI: 10.1002/cbic.201200253

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 12, pages 1750–1753, August 13, 2012

Additional Information

How to Cite

Neef, A. B., Samain, F. and Luedtke, N. W. (2012), Metabolic Labeling of DNA by Purine Analogues in Vivo. ChemBioChem, 13: 1750–1753. doi: 10.1002/cbic.201200253

Author Information

  1. Institute of Organic Chemistry, University of Zürich, Winterthurerstrasse 190, 8057 Zürich (Switzerland)

*Institute of Organic Chemistry, University of Zürich, Winterthurerstrasse 190, 8057 Zürich (Switzerland)

Publication History

  1. Issue published online: 3 AUG 2012
  2. Article first published online: 20 JUL 2012
  3. Manuscript Received: 16 APR 2012

Funded by

  • Swiss National Science Foundation. Grant Number: 130074
  • Novartis Foundation. Grant Number: 37131903
  • University of Zürich Forschungskredit. Grant Number: 57131902

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Keywords:

  • click chemistry;
  • DNA replication;
  • imaging agents;
  • metabolic labeling;
  • purine nucleosides
Thumbnail image of graphical abstract

DNA tracking: The 2′-deoxyadenosine analogue “EdA” efficiently labels newly synthesized DNA with alkyne groups in vivo without inhibiting cell cycle progression or embryonic development. This provides the first demonstration that a bio-orthogonal functional group can be metabolically incorporated into DNA through endogenous pathways by addition of a purine analogue to cells.

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