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Communication

A Single-Cell NMR Membrane Transport Assay

  1. Prof. Dr. Werner Kremer1,*,
  2. Prof. Dr. Hans Robert Kalbitzer1,
  3. Dr. Christina Schreier2,
  4. Dr. Fritz Huber2,
  5. Christine Schulze3,
  6. Prof. Dr. Hannelore Daniel3,
  7. Dr. Jürgen Stolz3,*

Article first published online: 23 OCT 2012

DOI: 10.1002/cbic.201200514

Issue

ChemBioChem

ChemBioChem

Volume 13, Issue 17, pages 2501–2504, November 26, 2012

Additional Information

How to Cite

Kremer, W., Kalbitzer, H. R., Schreier, C., Huber, F., Schulze, C., Daniel, H. and Stolz, J. (2012), A Single-Cell NMR Membrane Transport Assay. ChemBioChem, 13: 2501–2504. doi: 10.1002/cbic.201200514

Author Information

  1. 1

    Institut für Biophysik und Physikalische Biochemie und Zentrum für Magnetische Resonanz in Chemie und Biomedizin, Universität Regensburg, Universitätstrasse 31, 93053 Regensburg (Germany)

  2. 2

    LipoFIT Analytic GmbH, Josef-Engert-Str. 9, 93053 Regensburg (Germany)

  3. 3

    Lehrstuhl für Ernährungsphysiologie, Zentralinstitut für Ernährungs- und Lebensmittelforschung (ZIEL)—, Abteilung Biochemie, Wissenschaftszentrum Weihenstephan, Technische Universität München, Gregor-Mendel-Strasse 2, 85350 Freising (Germany)

*Institut für Biophysik und Physikalische Biochemie und Zentrum für Magnetische Resonanz in Chemie und Biomedizin, Universität Regensburg, Universitätstrasse 31, 93053 Regensburg (Germany)

Publication History

  1. Issue published online: 17 NOV 2012
  2. Article first published online: 23 OCT 2012
  3. Manuscript Received: 8 AUG 2012

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Keywords:

  • amino acids;
  • NMR spectroscopy;
  • peptide transport;
  • plasma membranes;
  • single-cell NMR
Thumbnail image of graphical abstract

Peptide transport: We describe a generally applicable NMR-based assay that can detect transport processes across the plasma membrane on a single-cell level. Single-cell 1D 1H NMR spectroscopy clearly demonstrated the presence of transported substrates inside the cells. NMR-based measurements involving single cRNA-injected oocytes might provide an ab initio method for searching for transported substrates.

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