SEARCH

SEARCH BY CITATION

Keywords:

  • biosynthesis;
  • C[BOND]C coupling;
  • heme proteins;
  • indolocarbazoles;
  • staurosporine
Thumbnail image of graphical abstract

Central C[BOND]C coupling: It has been proposed that the heme-containing enzyme StaD and its homologues catalyze a coupling reaction that yields an indolepyruvic acid (IPA) imine in indolocarbazole biosynthesis. We show that StaD uses IPA enol as an analogue of natural substrate to produce an IPA dimer, in the first direct evidence of the C[BOND]C coupling dimerization reaction catalyzed by StaD.