These authors contributed equally to this work.
Caged CO2 for the Direct Observation of CO2-Consuming Reactions
Article first published online: 16 JAN 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 14, Issue 3, pages 372–380, February 11, 2013
How to Cite
Lommel, K., Schäfer, G., Grenader, K., Ruland, C., Terfort, A., Mäntele, W. and Wille, G. (2013), Caged CO2 for the Direct Observation of CO2-Consuming Reactions. ChemBioChem, 14: 372–380. doi: 10.1002/cbic.201200659
- Issue published online: 6 FEB 2013
- Article first published online: 16 JAN 2013
- Manuscript Received: 15 OCT 2012
- Goethe University Frankfurt
- caged CO2;
- enzyme catalysis;
- IR spectroscopy;
CO2-consuming reactions, in particular carboxylations, play important roles in technical processes and in nature. Their kinetic behavior and the reaction mechanisms of carboxylating enzymes are difficult to study because CO2 is inconvenient to handle as a gas, exists in equilibrium with bicarbonate in aqueous solution, and typically yields products that show no significant spectroscopic differences from the reactants in the UV/Vis range. Here we demonstrate the utility of 3-nitrophenylacetic acid and related compounds (caged CO2) in conjunction with infrared spectroscopy as widely applicable tools for the investigation of such reactions, permitting convenient measurement of the kinetics of CO2 consumption. The use of isotopically labeled caged CO2 provides a tool for the assignment of infrared absorption bands, thus aiding insight into reaction intermediates and mechanisms.