Fluorescent Live-Cell Imaging of Metabolically Incorporated Unnatural Cyclopropene-Mannosamine Derivatives

Authors

  • Christian M. Cole,

    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093 (USA)
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    • These authors contributed equally to this work.

  • Dr. Jun Yang,

    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093 (USA)
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    • These authors contributed equally to this work.

  • Dr. Jolita Šečkutė,

    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093 (USA)
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  • Prof. Neal K. Devaraj

    Corresponding author
    1. Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093 (USA)
    • Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093 (USA)
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Abstract

original image

Sugar coated: We recently developed methylcyclopropenes as low-molecular-weight tetrazine coupling partners. Here, we demonstrate that methylcyclopropenes can meet the stringent steric demands required for metabolic imaging of unnatural mannosamines on live cells. Using sequential azide–alkyne chemistry, we also demonstrate multicolor imaging of two different metabolically incorporated unnatural sugars.

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