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Tetramolecular Fluorescence Complementation for Detection of Specific RNAs in Vitro

Authors

  • Stefanie Julia Kellermann,

    1. University of Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology, Martin Luther King Platz 6, 20146 Hamburg (Germany)
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    • These authors contributed equally to this work.

  • Anna Katharina Rath,

    1. University of Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology, Martin Luther King Platz 6, 20146 Hamburg (Germany)
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    • These authors contributed equally to this work.

  • Dr. Andrea Rentmeister

    Corresponding author
    1. University of Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology, Martin Luther King Platz 6, 20146 Hamburg (Germany)
    2. The Hamburg Centre for Ultrafast Imaging, Luruper Chaussee 149, 22761 Hamburg (Germany)
    • University of Hamburg, Department of Chemistry, Institute of Biochemistry and Molecular Biology, Martin Luther King Platz 6, 20146 Hamburg (Germany)
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Abstract

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Short fuses: RNA can be detected sequence specifically by using two RNA binding proteins fused to short strands derived from GFP and an additional large GFP fragment. The tetramolecular system has a high signal-to-noise ratio, discriminates between closely related RNA, and works in RNA preparations as well as E. coli cell lysate.

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