Selective Isotope Labelling of Leucine Residues by Using α-Ketoacid Precursor Compounds



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You can have one without the other: A new metabolic precursor compound can selectively introduce 13C and 2H patterns at leucine residues in proteins in cell-based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high-molecular-weight proteins by NMR spectroscopy.