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Insights into TIM-Barrel Prenyl Transferase Mechanisms: Crystal Structures of PcrB from Bacillus subtilis and Staphylococcus aureus

Authors

  • Feifei Ren,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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    • These authors contributed equally to this work.

  • Xinxin Feng,

    1. Department of Chemistry, University of Illinois, 600 South Mathews Ave. Urbana, IL 61801 (USA)
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    • These authors contributed equally to this work.

  • Dr. Tzu-Ping Ko,

    1. Institute of Biological Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529 (Taiwan)
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    • These authors contributed equally to this work.

  • Dr. Chun-Hsiang Huang,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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  • Yumei Hu,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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  • Dr. Hsiu-Chien Chan,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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  • Yi-Liang Liu,

    1. Department of Chemistry, University of Illinois, 600 South Mathews Ave. Urbana, IL 61801 (USA)
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  • Dr. Ke Wang,

    1. Department of Chemistry, University of Illinois, 600 South Mathews Ave. Urbana, IL 61801 (USA)
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  • Dr. Chun-Chi Chen,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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  • Xuefei Pang,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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  • Miao He,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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  • Yujie Li,

    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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  • Prof. Dr. Eric Oldfield,

    Corresponding author
    1. Department of Chemistry, University of Illinois, 600 South Mathews Ave. Urbana, IL 61801 (USA)
    • Department of Chemistry, University of Illinois, 600 South Mathews Ave. Urbana, IL 61801 (USA)
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  • Prof. Dr. Rey-Ting Guo

    Corresponding author
    1. Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
    • Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 XiQiDao, Tianjin 300308 (China)
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Abstract

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Well structured: As a new triose phosphate isomerase (TIM) barrel-fold prenyl transferase, PcrB catalyzes the production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate. Crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus in complex with ligands were solved, and together with site-directed mutagenesis and bioinformatics analyses, clearly reveal the catalytic mechanism of the enzyme.

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