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Keywords:

  • click chemistry;
  • fluorescent probes;
  • photoaffinity labeling;
  • proteomics;
  • small molecule–protein interactions
Thumbnail image of graphical abstract

Photo opportunity: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.