Selective Fluorescence Detection of Small-Molecule-Binding Proteins by Using a Dual Photoaffinity Labeling System

Authors

  • Prof. Kaori Sakurai,

    Corresponding author
    1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588 (Japan)
    • Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588 (Japan)
    Search for more papers by this author
  • Rika Yamada,

    1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588 (Japan)
    Search for more papers by this author
  • Ayumi Okada,

    1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588 (Japan)
    Search for more papers by this author
  • Masaki Tawa,

    1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588 (Japan)
    Search for more papers by this author
  • Shimpei Ozawa,

    1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588 (Japan)
    Search for more papers by this author
  • Maia Inoue

    1. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Naka-cho, 2-24-16, Koganei, Tokyo, 184-8588 (Japan)
    Search for more papers by this author

Abstract

original image

Photo opportunity: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.

Ancillary