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Keywords:

  • cobalamin enzymes;
  • enzyme mechanism;
  • radical SAM methyltransferase;
  • tryptophan methyltransferase
Thumbnail image of graphical abstract

All about Me: Pierre and co-workers have revealed mechanistic details of a tryptophan methyltransferase (TsrM) involved in the biosynthesis of the thiopeptide antibiotic, thiostrepton. Utilising cobalamin and a [4Fe–4S] cluster to generate 2-methyltryptophan from tryptophan, a key difference between this enzyme and other radical SAM methyltransferases is that the reaction is not initiated by a single-electron reduction of SAM to generate 5′-dA.