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Structure and Biosynthesis of Fimsbactins A–F, Siderophores from Acinetobacter baumannii and Acinetobacter baylyi

Authors

  • Anna Proschak,

    1. Fachbereich Biowissenschaften, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany)
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  • Patrice Lubuta,

    1. Fachbereich Biowissenschaften, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany)
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  • Peter Grün,

    1. Fachbereich Biowissenschaften, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany)
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  • Dr. Frank Löhr,

    1. Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue Strasse 9, 60438 Frankfurt am Main (Germany)
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  • Dr. Gottfried Wilharm,

    1. Robert Koch Institute, Wernigerode Branch, Burgstrasse 37, 38855 Wernigerode (Germany)
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  • Veronique De Berardinis,

    1. CEA, DSV, IG, Genoscope and CNRS-UMR8030, 2 rue Gaston Crémieux, 91057 Evry (France)
    2. Université d'Evry Val d'Essonne, Boulevard François Mitterrand, 91057 Evry (France)
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  • Prof. Dr. Helge B. Bode

    Corresponding author
    1. Fachbereich Biowissenschaften, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany)
    • Fachbereich Biowissenschaften, Goethe University Frankfurt, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main (Germany)
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Abstract

Novel chatechol/hydroxamate siderophores (named “fimsbactins”) were identified in Acinetobacter baumannii ATCC 17978 and Acinetobacter baylyi ADP1. The major compound, fimsbactin A, was isolated from low-iron cultures of A. baylyi ADP1, and its chemical structure was elucidated by mass spectrometry, and detailed 1H, 13C and 15N NMR spectroscopy. From inverse feeding experiments following HPLC-MS analysis, the structures of five additional derivatives were elucidated. The gene cluster encoding the fimsbactin synthetase (fbs) was identified in both genomes, and mutants in fbs genes in A. baylyi were analyzed, thus allowing prediction of the fimsbactin biosynthesis pathway.

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