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Keywords:

  • endonucleases;
  • exonucleases;
  • oligonucleotides;
  • pyrene;
  • quenching;
  • sensors
Thumbnail image of graphical abstract

Nuclease sensor trio: Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3′-exonucleases, 5′-exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media.