Synthesis of Heterocyclic Terpenoids by Promiscuous Squalene–Hopene Cyclases



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Promiscuous enzymes: The substrate promiscuity of squalene–hopene cyclases has been explored and applied in the enzyme-catalyzed synthesis of heterocyclic terpenoids. Features of this work include cyclization reactions without pyrophosphate activation, and stereospecific ring closure of substrates of varying chain length and terminal nucleophile. This provides a biocatalytic alternative to traditional chemical catalysts.