An in Vivo Tagging Method Reveals that Ras Undergoes Sustained Activation upon Transglutaminase-Mediated Protein Serotonylation

Authors

  • Jason Ching-Yao Lin,

    1. Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    2. Department of Chemistry, National Tsing Hua University, 101 Kuang-Fu Road Section 2, Hsinchu 30013 (Taiwan)
    3. Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
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    • These authors contributed equally to this work.

  • Dr. Chi-Chi Chou,

    1. Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    2. Core Facilities for Protein Structural Analysis, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
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    • These authors contributed equally to this work.

  • Dr. Shijay Gao,

    1. Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
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    • These authors contributed equally to this work.

  • Shang-Chuen Wu,

    1. Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    2. Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan)
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  • Prof. Kay-Hooi Khoo,

    1. Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    2. Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    3. Core Facilities for Protein Structural Analysis, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    4. Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan)
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  • Prof. Chun-Hung Lin

    Corresponding author
    1. Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    2. Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)
    3. Institute of Biochemical Sciences, National Taiwan University, 1 Roosevelt Road Section 4, Taipei 10617 (Taiwan)
    • Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529 (Taiwan)

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Abstract

original image

Don't interrupt! Protein serotonylation has been implicated in living cells, yet its role remains poorly defined because of the lack of characterization tools. We synthesized a serotonin derivative to enable selective tagging of serotonylation and to investigate its effect on Ras; the latter displayed undisrupted interaction with Raf-1 at the Ras binding domain.

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