Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays

Authors

  • Dr. Sonia Serna,

    1. Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, Parque Tecnológico, 20009 San Sebastian (Spain)
    Search for more papers by this author
  • Dr. Cornelis H. Hokke,

    1. Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden (The Netherlands)
    Search for more papers by this author
  • Dr. Martin Weissenborn,

    1. The School of Chemistry and MIB, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
    Search for more papers by this author
  • Prof. Dr. Sabine Flitsch,

    1. The School of Chemistry and MIB, The University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
    Search for more papers by this author
  • Prof. Dr. Manuel Martin-Lomas,

    1. Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, Parque Tecnológico, 20009 San Sebastian (Spain)
    2. CIBER BBN, P° de Miramón 182, Parque Tecnológico, 20009 San Sebastian (Spain)
    Search for more papers by this author
  • Dr. Niels-Christian Reichardt

    Corresponding author
    1. Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, Parque Tecnológico, 20009 San Sebastian (Spain)
    2. CIBER BBN, P° de Miramón 182, Parque Tecnológico, 20009 San Sebastian (Spain)
    • Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, Parque Tecnológico, 20009 San Sebastian (Spain)

    Search for more papers by this author

Abstract

original image

High-throughput microarray technology has been combined with ultrasensitive and high-resolution tritium autoradiography to create a new platform for the quantitative detection of glycosyltransferase activity on glycan arrays. In addition, we show full compatibility with the use of fluorescently labeled lectins to help with the stereochemical assignment of newly formed glycoside linkages.

Ancillary