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Keywords:

  • amyloid fibrils;
  • intrinsic fluorescence;
  • kinetic assays;
  • protein aggregation
Thumbnail image of graphical abstract

Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein (blue curve) but not when α-synuclein seeds are incubated with β-synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case.