SEARCH

SEARCH BY CITATION

Keywords:

  • acyl transfer;
  • cyclotides;
  • native chemical ligation;
  • peptides;
  • protein engineering
Thumbnail image of graphical abstract

A selective N[RIGHTWARDS ARROW]S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.