• Open Access

Making Ends Meet: Chemically Mediated Circularization of Recombinant Proteins

Authors

  • Dr. Ben Cowper,

    1. Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London, WC1H 0AJ (UK)
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  • Prof. Dr. David J. Craik,

    1. Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland, 4072 (Australia)
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  • Dr. Derek Macmillan

    Corresponding author
    1. Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London, WC1H 0AJ (UK)
    • Department of Chemistry, University College London, Christopher Ingold Building, 20 Gordon Street, London, WC1H 0AJ (UK)

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Abstract

original image

A selective N→S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements.

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