Fluorescent THF-Based Fructose Analogue Exhibits Fructose-Dependent Uptake

Authors

  • Dr. Marina Tanasova,

    1. Department of Health Sciences and Technology, Swiss Federal Institute of Technology (ETH Zürich), Schmelzbergstrasse 9, 8092 Zürich (Switzerland)
    Search for more papers by this author
  • Matthew Plutschack,

    1. Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390 (USA)
    Search for more papers by this author
  • Megan E. Muroski,

    1. Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390 (USA)
    Search for more papers by this author
  • Prof. Shana J. Sturla,

    1. Department of Health Sciences and Technology, Swiss Federal Institute of Technology (ETH Zürich), Schmelzbergstrasse 9, 8092 Zürich (Switzerland)
    Search for more papers by this author
  • Prof. Geoffrey F. Strouse,

    1. Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390 (USA)
    Search for more papers by this author
  • Prof. D. Tyler McQuade

    Corresponding author
    1. Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390 (USA)
    2. Department for Biomolecular Systems, Max Planck Institute for Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam (Germany)
    • Department of Chemistry and Biochemistry, Florida State University, 95 Chieftan Way, Tallahassee, FL 32306-4390 (USA)===

    Search for more papers by this author

Abstract

Recent publications suggest that high dietary fructose might play a significant role in cancer metabolism and can exacerbate a number of aspects of metabolic syndrome. Addressing the role that fructose plays in human health is a controversial question and requires a detailed understanding of many factors including the mechanism of fructose transport into healthy and diseased cells. Fructose transport into cells is thought to be largely mediated by the passive hexose transporters Glut2 and Glut5. To date, no probes that can be selectively transported by one of these enzymes but not by the other have been identified. The data presented here indicate that, in MCF-7 cells, a 1-amino-2,5-anhydro-D-mannitol-based fluorescent NBDM probe is transported twice as efficiently as fructose and that this takes place with the aid of Glut5. Its Glut5 specificity and differential uptake in cancer cells and in normal cells suggest this NBDM probe as a potentially useful tool for cross-cell-line correlation of Glut5 transport activity.

Ancillary