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Keywords:

  • bioorthogonal reaction;
  • cycloaddition;
  • fluorescence labeling;
  • methyltransferase;
  • protein methylation;
  • S-adenosyl-L-methionine
Thumbnail image of graphical abstract

Protein allylation and fluorophore targeting: Arginine residues of the yeast nuclear ribonucleoprotein Npl3 were extensively modified by Hmt1-catalyzed allylation reaction with allyl-SAM as the allyl group donor. The allylated protein was further treated with tetrazole compounds under UV irradiation, leading to formation of protein-attached fluorescent products.