SEARCH

SEARCH BY CITATION

Keywords:

  • biosynthesis;
  • colibactin;
  • E. coli Nissle;
  • gene expression;
  • prodrug maturation
Thumbnail image of graphical abstract

Releasing the cytopath: We have identified an N-myristoyl-D-asparagine (1) as the free N-terminal prodrug scaffold in cytopathogenic Escherichia coli strains expressing the colibactin gene cluster. Colibactin is released in vivo upon cleavage of precolibactin. We provide for the first time in vivo evidence of the prodrug-like release mechanism of colibactin.