DNA Binding and Bending by Sac7d is Stepwise

Authors

  • Dr. Justin Spiriti,

    1. Department of Chemistry, University of South Florida, 4202 E. Fowler Ave. CHE 205, Tampa, FL 33620 (USA)
    2. Currently at Department of Computational & Systems Biology, School of Medicine, University of Pittsburgh, 3501 5th Ave. Suite 3064, Biomedical Science Tower 3 (BST3), Pittsburgh, PA 15260 (USA)
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  • Prof. Arjan van der Vaart

    Corresponding author
    1. Department of Chemistry, University of South Florida, 4202 E. Fowler Ave. CHE 205, Tampa, FL 33620 (USA)
    • Department of Chemistry, University of South Florida, 4202 E. Fowler Ave. CHE 205, Tampa, FL 33620 (USA)
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Abstract

original image

Which came first? Using thermodynamic cycles constructed from experimental measures of the binding free energy and free energy simulations, we have shown that binding of Sulfolobus acidocaldarius protein Sac7d to DNA occurs before DNA bending, thus indicating that a conformational selection mechanism is unlikely to be operative in Sac7d binding.

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