• Open Access

Production of Propane and Other Short-Chain Alkanes by Structure-Based Engineering of Ligand Specificity in Aldehyde-Deformylating Oxygenase

Authors

  • Dr. Basile Khara,

    1. Manchester Institute of Biotechnology and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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    • These authors contributed equally to this work.

  • Navya Menon,

    1. Manchester Institute of Biotechnology and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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    • These authors contributed equally to this work.

  • Dr. Colin Levy,

    1. Manchester Institute of Biotechnology and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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    • These authors contributed equally to this work.

  • Dr. David Mansell,

    1. Manchester Institute of Biotechnology and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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  • Debasis Das,

    1. Department of Chemistry, University of Michigan, 930 N. University, Ann Arbor, MI 48109 (USA)
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  • Prof. E. Neil G. Marsh,

    1. Department of Chemistry, University of Michigan, 930 N. University, Ann Arbor, MI 48109 (USA)
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  • Prof. David Leys,

    1. Manchester Institute of Biotechnology and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
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  • Prof. Nigel S. Scrutton

    Corresponding author
    1. Manchester Institute of Biotechnology and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)
    • Manchester Institute of Biotechnology and Faculty of Life Sciences, University of Manchester, 131 Princess Street, Manchester M1 7DN (UK)===

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Abstract

original image

Biocatalytic propane production: structure-based engineering of aldehyde-deformylating oxygenase improves specificity for short- and medium-chain-length aldehydes and enhances the propane generation in whole-cell biotransformations. This presents new opportunities for developing biocatalytic modules for the production of volatile “drop-in” biofuels.

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