The Crosslink Formation of 2′-OMe Oligonucleotide Containing 2-Amino-6-vinylpurine Protects mRNA from miRNA-Mediated Silencing

Authors

  • Dr. Shinya Hagihara,

    1. Institute of Transformative Bio-molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602 (Japan)
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  • Wei-Chen Lin,

    1. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577 (Japan)
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  • Shuhei Kusano,

    1. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577 (Japan)
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  • Xiao-guang Chao,

    1. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577 (Japan)
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  • Tsuneaki Hori,

    1. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577 (Japan)
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  • Dr. Shuhei Imoto,

    1. Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda Kumamoto 860-0082 (Japan)
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  • Prof. Fumi Nagatsugi

    Corresponding author
    1. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577 (Japan)
    • Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577 (Japan)
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Abstract

original image

Masking the miRNA binding site: Crosslink-forming oligonucleotide (CFO) was used for target gene-specific inhibition of microRNA (miRNA) functions. This method can interfere with specific miRNA–mRNA interactions by recognizing sequences unique to the 3′-UTR that are inherent in each mRNA.

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