An Isotopically Tagged Azobenzene-Based Cleavable Linker for Quantitative Proteomics

Authors

  • Dr. Yu Qian,

    1. Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467 (USA)
    Search for more papers by this author
  • Julianne Martell,

    1. Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467 (USA)
    Search for more papers by this author
  • Nicholas J. Pace,

    1. Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467 (USA)
    Search for more papers by this author
  • Dr. T. Eric Ballard,

    1. Neuroscience Medicinal Chemistry and Chemical Biology, Pfizer Worldwide Research and Development, 700 Main Street, Cambridge, MA 02139 (USA)
    Search for more papers by this author
  • Dr. Douglas S. Johnson,

    Corresponding author
    1. Neuroscience Medicinal Chemistry and Chemical Biology, Pfizer Worldwide Research and Development, 700 Main Street, Cambridge, MA 02139 (USA)
    • Neuroscience Medicinal Chemistry and Chemical Biology, Pfizer Worldwide Research and Development, 700 Main Street, Cambridge, MA 02139 (USA)
    Search for more papers by this author
  • Prof. Eranthie Weerapana

    Corresponding author
    1. Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467 (USA)
    • Department of Chemistry, Boston College, 2609 Beacon Street, Chestnut Hill, MA 02467 (USA)
    Search for more papers by this author

Abstract

original image

Putting a number on it: Cleavable linkers are widely utilized in proteomics applications. In particular, the azobenzene-based linker cleaves under mild conditions that are mass-spectrometry-compatible. Here, we adapt this linker for quantitative proteomic applications by incorporating an isotopic label. These light- and heavy-tagged linkers enable the identification and quantitation of labeled peptides from multiple proteomes.

Ancillary