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Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins

Authors

  • Dr. Sabine Schneider,

    Corresponding author
    1. Department of Chemistry, TU Munich, Lichtenbergstrasse 4, 85748 Garching (Germany)
    • Sabine Schneider, Department of Chemistry, TU Munich, Lichtenbergstrasse 4, 85748 Garching (Germany)

      Thomas Carell, Department of Chemistry, Ludwig-Maximilians University, Butenadtstrasse 5–13, 81377 Munich (Germany)

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  • Dipl. Chem. Michael J. Gattner,

    1. Department of Chemistry, Ludwig-Maximilians University, Butenadtstrasse 5–13, 81377 Munich (Germany)
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  • Dr. Milan Vrabel,

    1. Department of Chemistry, Ludwig-Maximilians University, Butenadtstrasse 5–13, 81377 Munich (Germany)
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  • MSc Veronika Flügel,

    1. Department of Chemistry, TU Munich, Lichtenbergstrasse 4, 85748 Garching (Germany)
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  • Dr. Verónica López-Carrillo,

    1. Department of Chemistry, Ludwig-Maximilians University, Butenadtstrasse 5–13, 81377 Munich (Germany)
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  • Dr. Stefan Prill,

    1. Department of Chemistry, Ludwig-Maximilians University, Butenadtstrasse 5–13, 81377 Munich (Germany)
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  • Prof. Dr. Thomas Carell

    Corresponding author
    1. Department of Chemistry, Ludwig-Maximilians University, Butenadtstrasse 5–13, 81377 Munich (Germany)
    • Sabine Schneider, Department of Chemistry, TU Munich, Lichtenbergstrasse 4, 85748 Garching (Germany)

      Thomas Carell, Department of Chemistry, Ludwig-Maximilians University, Butenadtstrasse 5–13, 81377 Munich (Germany)

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Abstract

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Three for two: By using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase.

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