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Measurement by SPR of Very Low Dissociation Rates: Oxidation-Mediated Loss of Biotin–Streptavidin Affinity

Authors

  • Dr. Mario A. E. Rebhan,

    1. Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Wolfgang-Pauli Strasse 10, 8093 Zürich (Switzerland)
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  • Dr. Andreas Brunschweiger,

    1. Faculty of Chemistry and Chemical Biology, TU Dortmund University, Otto-Hahn-Strasse 6, 44227 Dortmund (Germany)
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  • Prof. Dr. Jonathan Hall

    Corresponding author
    1. Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Wolfgang-Pauli Strasse 10, 8093 Zürich (Switzerland)
    • Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, Wolfgang-Pauli Strasse 10, 8093 Zürich (Switzerland)

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Abstract

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Long-term relationship: Biotin labels on RNAs, and possibly other biomacromolecules, are easily oxidized causing a dramatic loss of affinity for streptavidin and adversely affecting the measurement of high-affinity interactions. A new SPR method has been developed for measuring the very low rate-dissociation constants of biotin- and biotin oxide-conjugated RNAs with streptavidin.

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