Get access

A Fluorogenic Probe for γ-Glutamyl Cyclotransferase: Application of an Enzyme-Triggered O-to-N Acyl Migration-Type Reaction

Authors

  • Dr. Taku Yoshiya,

    Corresponding author
    1. SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan)
    • SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan)

    Search for more papers by this author
  • Shugo Tsuda,

    1. SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan)
    Search for more papers by this author
  • Masayoshi Mochizuki,

    1. SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan)
    Search for more papers by this author
  • Dr. Koushi Hidaka,

    1. Faculty of Pharmaceutical Sciences, Cooperative Research Center of Life Sciences, Kobe Gakuin University, 1-1-3 Minatojima, Chuo-ku, Kobe 650-8586 (Japan)
    Search for more papers by this author
  • Prof. Dr. Yuko Tsuda,

    1. Faculty of Pharmaceutical Sciences, Cooperative Research Center of Life Sciences, Kobe Gakuin University, 1-1-3 Minatojima, Chuo-ku, Kobe 650-8586 (Japan)
    Search for more papers by this author
  • Prof. Dr. Yoshiaki Kiso,

    1. Center for Frontier Research in Medicinal Science, Kyoto Pharmaceutical University, 1 Misasagi-Shichonocho, Yamashina-ku, Kyoto 607-8412 (Japan)
    2. Laboratory of Peptide Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama 526-0829 (Japan)
    Search for more papers by this author
  • Dr. Susumu Kageyama,

    1. Department of Urology, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu 520-2192 (Japan)
    Search for more papers by this author
  • Dr. Hiromi Ii,

    1. Department of Clinical Oncology, Kyoto Pharmaceutical University, 1 Misasagi-Shichonocho, Yamashina-ku, Kyoto 607-8412 (Japan)
    Search for more papers by this author
  • Prof. Dr. Tatsuhiro Yoshiki,

    1. Department of Clinical Oncology, Kyoto Pharmaceutical University, 1 Misasagi-Shichonocho, Yamashina-ku, Kyoto 607-8412 (Japan)
    Search for more papers by this author
  • Prof. Dr. Yuji Nishiuchi

    Corresponding author
    1. SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan)
    2. Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka 560-0043 (Japan)
    • SAITO Research Center, Peptide Institute, Inc. 7-2-9 Saito-Asagi, Ibaraki, Osaka 567-0085 (Japan)

    Search for more papers by this author

Abstract

original image

Light it up: Human chromosome 7 ORF 24, a tumor-related protein, has been identified as a γ-glutamyl cyclotransferase (GGCT) in the glutathione homeostasis cycle. The singular substrate preference of the enzyme has hampered chemical probe development, and no fluorogenic probe has been reported. Here we report the first fluorogenic dipeptide probe, LISA-4, which should contribute toward further understanding of GGCT.

Ancillary