Cell-Free Preparation of Functional and Triggerable Giant Proteoliposomes

Authors

  • Dr. Yan-Jun Liu,

    1. Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris (France) http://www.baigllab.com/
    2. Université Pierre et Marie Curie Paris 6, 4 place Jussieu, 75005 Paris (France)
    3. UMR 8640, CNRS, 3 rue Michel-Ange, 75016 Paris (France)
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  • Gregory P. R. Hansen,

    1. Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris (France) http://www.baigllab.com/
    2. Université Pierre et Marie Curie Paris 6, 4 place Jussieu, 75005 Paris (France)
    3. UMR 8640, CNRS, 3 rue Michel-Ange, 75016 Paris (France)
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  • Anna Venancio-Marques,

    1. Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris (France) http://www.baigllab.com/
    2. Université Pierre et Marie Curie Paris 6, 4 place Jussieu, 75005 Paris (France)
    3. UMR 8640, CNRS, 3 rue Michel-Ange, 75016 Paris (France)
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  • Prof. Damien Baigl

    Corresponding author
    1. Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris (France) http://www.baigllab.com/
    2. Université Pierre et Marie Curie Paris 6, 4 place Jussieu, 75005 Paris (France)
    3. UMR 8640, CNRS, 3 rue Michel-Ange, 75016 Paris (France)
    • Department of Chemistry, Ecole Normale Superieure, 24 rue Lhomond, 75005 Paris (France) http://www.baigllab.com/

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Abstract

original image

Heat, we leak: We express a membrane protein outside well-defined giant liposomes obtained by gravity-transferred sucrose-in-oil droplets into a cell-free, reconstituted expression system. We show that the presence of the liposome is necessary during expression for efficient protein insertion into the membrane and that temperature can trigger the resulting membrane function.

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