Cloning and Sequencing of the Chaetocin Biosynthetic Gene Cluster

Authors

  • Dr. Thomas Gerken,

    1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115 (USA)
    2. Current address: Metanomics GmbH, Tegeler Weg 33, 10589 Berlin (Germany)
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  • Prof. Christopher T. Walsh

    Corresponding author
    1. Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115 (USA)
    • Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115 (USA)

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Abstract

original image

How a fungus does it: The epidithiodiketopiperazine (ETP) fungal alkaloids, which include gliotoxin, have garnered much attention since their initial isolation. The cloning and sequencing of the biosynthetic gene cluster for another ETP, chaetocin A (see figure) were used to suggest a route towards dimerization and sulfur incorporation in these molecules.

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