These authors contributed equally to this work.
Biosynthesis of Hygrocins, Antitumor Naphthoquinone Ansamycins Produced by Streptomyces sp. LZ35
Article first published online: 25 NOV 2013
Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 15, Issue 1, pages 94–102, January 3, 2014
How to Cite
Li, S., Wang, H., Li, Y., Deng, J., Lu, C., Shen, Y. and Shen, Y. (2014), Biosynthesis of Hygrocins, Antitumor Naphthoquinone Ansamycins Produced by Streptomyces sp. LZ35. ChemBioChem, 15: 94–102. doi: 10.1002/cbic.201300599
- Issue published online: 19 DEC 2013
- Article first published online: 25 NOV 2013
- Manuscript Received: 17 SEP 2013
- National Natural Science Foundation of China. Grant Number: 31100079
- 973 Programs. Grant Numbers: 2010CB833802, 2012CB721005
- IIFSDU. Grant Number: 2013GN014
- Baeyer–Villiger monooxygenases;
- luciferase-like monooxygenases
Hygrocins are naphthoquinone ansamycins with significant antitumor activities. Here, we report the identification and characterization of the hygrocin biosynthetic gene cluster (hgc) in Streptomyces sp. LZ35. A biosynthetic pathway is proposed based on bioinformatics analysis of the hgc genes and intermediates accumulated in selected gene disruption mutants. One of the steps during the biosynthesis of hygrocins is a Baeyer–Villiger oxidation between C5 and C6, catalyzed by luciferase-like monooxygenase homologue Hgc3. Hgc3 represents the founding member of a previously uncharacterized family of enzymes acting as Baeyer–Villiger monooxygenases.