Get access

Chemoenzymatic Bio-orthogonal Chemistry for Site-Specific Double Modification of Recombinant Thrombomodulin

Authors

  • Dr. Rui Jiang,

    1. Department of Chemistry, Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Ave, Cleveland, OH 44115 (USA)
    Search for more papers by this author
  • Lin Wang,

    1. Department of Chemistry, Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Ave, Cleveland, OH 44115 (USA)
    Search for more papers by this author
  • Dr. Jacob Weingart,

    1. Department of Chemistry, Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Ave, Cleveland, OH 44115 (USA)
    Search for more papers by this author
  • Prof. Dr. Xue-Long Sun

    Corresponding author
    1. Department of Chemistry, Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Ave, Cleveland, OH 44115 (USA)
    • Department of Chemistry, Chemical and Biomedical Engineering, Cleveland State University, 2121 Euclid Ave, Cleveland, OH 44115 (USA)===

    Search for more papers by this author

Abstract

original image

Best of both worlds: A one-pot strategy for site-specific PEGylation through strain-promoted alkyne–azide cycloaddition (SPAAC) and fluorescent labeling through sortase A-mediated ligation (SML) of recombinant thrombomodulin without prior chemical modification and without diminishing the protein activity has been developed.

Ancillary