Structure Elucidation of DNA–Protein Crosslinks by Using Reductive Desulfurization and Liquid Chromatography–Tandem Mass Spectrometry

Authors

  • Susith Wickramaratne,

    1. University of Minnesota Masonic Cancer Center and the Department of Chemistry, 2231 6th Street SE, Room 2-220 CCRB, Minneapolis, MN 55455 (USA)
    Search for more papers by this author
  • Prof. Natalia Y. Tretyakova

    Corresponding author
    1. University of Minnesota Masonic Cancer Center and the Department of Medicinal Chemistry, 2231 6th Street SE, Room 2-147 CCRB, Minneapolis, MN 55455 (USA)
    • University of Minnesota Masonic Cancer Center and the Department of Medicinal Chemistry, 2231 6th Street SE, Room 2-147 CCRB, Minneapolis, MN 55455 (USA)

    Search for more papers by this author

Abstract

original image

Easier with ethyl: Guengerich and co-workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT–DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl–DNA adducts, which can be readily characterized by LC-MSn.

Ancillary