Inside Cover: Highly Efficient Förster Resonance Energy Transfer in a Fast, Serum-Compatible Immunoassay (ChemBioChem 6/2013)



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The inside cover picture shows the assay principle of a FRET-based homogeneous immunoassay described by T. Zuchner et al. on p. 699 ff. When using the novel phosphorescent dye EuLH and BHQ-10 as the FRET pair, the FRET efficiency in this homogeneous, microplate-based immunoassay increases to >99.5 %, thus leading to significantly improved signal-to-background ratio, precision and linear range.