Inside Cover: Effects of FlAsH/Tetracysteine (TC) Tag on PrP Proteolysis and PrPres Formation by TC-Scanning (ChemBioChem 13/2013)

Authors

  • Dr. Yuzuru Taguchi,

    Corresponding author
    1. Rocky Mountain Laboratories, NIAID, NIH, Laboratory of Persistent Viral Diseases, 903 S. 4th St., Hamilton, MT 59840 (USA)
    2. Currently at the Department of Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, T2N 4Z6 (Canada)
    • Rocky Mountain Laboratories, NIAID, NIH, Laboratory of Persistent Viral Diseases, 903 S. 4th St., Hamilton, MT 59840 (USA)===

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  • Lindsay A. Hohsfield,

    Corresponding author
    1. Rocky Mountain Laboratories, NIAID, NIH, Laboratory of Persistent Viral Diseases, 903 S. 4th St., Hamilton, MT 59840 (USA)
    2. Currently at the Laboratory of Psychiatry and Exp. Alzheimer's Research, Department of Psychiatry and Psychotherapy, Innsbruck Medical University, Anichstr. 35, 6020 Innsbruck (Austria)
    • Rocky Mountain Laboratories, NIAID, NIH, Laboratory of Persistent Viral Diseases, 903 S. 4th St., Hamilton, MT 59840 (USA)===

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  • Dr. Jason R. Hollister,

    1. Rocky Mountain Laboratories, NIAID, NIH, Laboratory of Persistent Viral Diseases, 903 S. 4th St., Hamilton, MT 59840 (USA)
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  • Dr. Gerald S. Baron

    Corresponding author
    1. Rocky Mountain Laboratories, NIAID, NIH, Laboratory of Persistent Viral Diseases, 903 S. 4th St., Hamilton, MT 59840 (USA)
    • Rocky Mountain Laboratories, NIAID, NIH, Laboratory of Persistent Viral Diseases, 903 S. 4th St., Hamilton, MT 59840 (USA)===

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Abstract

original image

The inside cover picture shows a mapping technique by Y. Taguchi, L. A. Hohsfield, G. S. Baron et al. (see p. 1597 ff.). Tetracysteine (TC) motifs bind fluorescent biarsenical dyes (FlAsH). Positions where FlAsH/TC insertion into PrP inhibits trypsin cleavage are detected by SDS-PAGE, thus localizing points of contact between PrP and trypsin. Thanks to Darrell Hurt (BCBB, NIAID) and Austin Athman for graphics assistance.

Cartoon 1.

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