An Expanded Set of Fluorogenic Sulfatase Activity Probes

Authors

  • Elizabeth L. Smith,

    1. Departments of Chemistry and Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, B84 Hildebrand Hall, #1460, Berkeley, CA 94720 (USA)
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  • Prof. Carolyn R. Bertozzi,

    1. Departments of Chemistry and Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, B84 Hildebrand Hall, #1460, Berkeley, CA 94720 (USA)
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  • Prof. Kimberly E. Beatty

    Corresponding author
    1. Department of Physiology and Pharmacology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, L334, Portland, OR 97239 (USA)
    • Department of Physiology and Pharmacology, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, L334, Portland, OR 97239 (USA)===

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Abstract

Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases.

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